Introduction:
How do we count bacteria? In this article, we will explore the methods used to determine the number of bacteria present in a sample.
1. Plate Count Method
The most common method for bacterial counting is the plate count method. In this method, a small amount of the sample is spread onto an agar plate and incubated under optimal conditions for bacterial growth. After incubation, the colonies that have formed on the plate are counted and multiplied by the dilution factor to obtain the total number of bacteria in the original sample.
2. Microscopic Count Method
Another method for bacterial counting is the microscopic count method. In this method, a small volume of the sample is placed on a microscope slide and viewed under a microscope. The bacteria are then counted using a special grid pattern on the slide. This method is faster than the plate count method, but it does not provide information about the viability of the bacteria.
3. Spectrophotometer Method
The spectrophotometer method is a rapid and non-destructive method for bacterial counting. In this method, the sample is placed in a spectrophotometer and the absorbance of light is measured. The absorbance is then compared to a standard curve to determine the concentration of bacteria in the sample.
Conclusion:
【细菌计数英文缩写细菌计数英文怎么说读】In conclusion, there are several methods available for bacterial counting, including the plate count method, microscopic count method, and spectrophotometer method. Each method has its advantages and disadvantages, and the choice of method depends on the specific needs of the experiment or application.
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